Computer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.

TitleComputer-Aided Laser Dissection: A Microdissection Workflow Leveraging Image Analysis Tools.
Publication TypeJournal Article
Year of Publication2018
AuthorsHipp, JD, Johann DJ, Chen Y, Madabhushi A, Monaco J, Cheng J, Rodriguez-Canales J, Stumpe MC, Riedlinger G, Rosenberg AZ, Hanson JC, Kunju LP, Emmert-Buck MR, Balis UJ, Tangrea MA
JournalJournal of pathology informatics
Volume9
Pagination45
Date Published2018
ISSN2229-5089
Abstract

The development and application of new molecular diagnostic assays based on next-generation sequencing and proteomics require improved methodologies for procurement of target cells from histological sections. Laser microdissection can successfully isolate distinct cells from tissue specimens based on visual selection for many research and clinical applications. However, this can be a daunting task when a large number of cells are required for molecular analysis or when a sizeable number of specimens need to be evaluated.

DOI10.4103/jpi.jpi_60_18
PDF Link

http://www.ncbi.nlm.nih.gov/pubmed/30622835?dopt=Abstract

Alternate JournalJ Pathol Inform

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