277th LG: Third-harmonic generation imaging of breast tissue biopsies.

Friday, July 1, 2016 - 10:45
German Corredor Prada
We demonstrate for the first time the imaging of unstained breast tissue biopsies using third-harmonic generation (THG) microscopy. As a label-free imaging technique, THG microscopy is compared to phase contrast and polarized light microscopy which are standard imaging methods forbreast tissues. A simple feature detection algorithm is applied to detect tumour-associated lymphocyte rich regions in unstained breast biopsytissue and compared with corresponding regions identified by a pathologist from bright-field images of hematoxylin and eosin stained breasttissue. Our results suggest that THG imaging holds potential as a complementary technique for analysing breast tissue biopsies.