The Nova NanoLab 200 FEG-SEM/FIB combines high resolution field emission scanning electron microscopy (SEM) and microanalysis with a focused ion beam (FIB) system for precise etching and metal deposition. It enables researchers to develop, create, modify, and characterize complex structures. It is a versatile nanotechnology laboratory tool for nanoscale prototyping, machining, 2D and 3D-characterization, and chemical microanalysis. Using the SPI mode allows a simultaneous patterning and imaging for real-time imaging feedback on the FIB milling process. It is equipped with an OmniProbe micromanipulator for TEM sample preparation.
The Nova NanoLab 200 enables characterization of both inorganic and biological samples via several methods, among them “Slice and View” for 3D-reconstruction, high resolution in-lens backscattered electron imaging for phase contrast characterization, and secondary ion imaging for microstructural analysis. In addition, it is equipped with a retractable solid state backscatter detector. It also has an integrated XEDS and EBSD Oxford system (XMax 50 mm EDS, Nordlys high resolution EBSD) which allows simultaneous collection of both chemical and orientation maps.
For biological applications, it enables simultaneous investigation of gross morphology of samples, cell surface characteristics, and subsurface structures. It also allows in situ manipulation of specimens to expose subsurface structures for SE and BSE imaging, or XEDS analysis to provide basic compositional, data as well as lamella liftout for further investigation with TEM or TOF-SIMS. A combination of imaging, micro-manipulation and compositional analysis techniques have been developed to explore structure–function relationships in macromolecular assemblies and cells. Three-dimensional (3D) imaging and reconstruction provides insight on the activities, functions, properties and organization of cells and a deeper understanding of the three-dimensional organization and function of cells.
It is a nanohistological tool for overall examination of cells and tissues under normal, stressed, or pathological conditions.