280th LG: Multi-Stain Analyser: An Automated Framework for Serial Section Registration and IHC Stain Analysis.

Friday, July 29, 2016 - 12:00
Nick Trahearn, PhD
Immunohistochemical (IHC) scoring plays a key role in many types of cancer pathology. Scoring is a visually estimated quantification of the reactivity of a given IHC marker, using qualities such as stain intensity and proportion of positivity. The scoring of these markers is known to be important in predicting response to treatment, especially targeted therapies. In histopathology, the need to section a tissue sample obfuscates the three dimensional relationships between adjacent serial sections. This is significant when when we wish to make multiple observations on the same sub-region of the tissue,  such as when studying the expression profile of several IHC stains on a localised region of interest.   Dr. Trahearn work has primarily been focused on these problems of IHC Stain Analysis and Serial Section Registration. The presentation will cover his work in these areas, as well as touching upon some of the key preprocessing steps that are performed before any registration or IHC analysis is undertaken, including automated Stain Separation and Out of Focus Slide detection. The main portion of the presentation will take the form of a case study of an IHC analysis project that he has worked on during the course of his PhD: The automated Allred Scoring of ER/PR stained breast core biopsies. During this portion of the talk Dr. Trahearn will detail the scoring framework and discuss some of the challenges that presented themselves over the course of the project. Dr. Trahearn will demonstrate that his ER/PR scoring algorithm is able to achieve good agreement with a pathologist, but also explore some of the cases where the pathologist and algorithm disagreed. Dr. Trahearn will conclude with an overview of his most recent work in this area: IHC analysis on colorectal cancer cases. The new project has many similar themes to the work on ER/PR scoring, but also many new challenges, including a much larger set of IHC markers. For this reason, certain adaptations were needed in order to accommodate the specific needs of the project. He will discuss some of the necessary adaptations and show some preliminary results from the project.