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Alsberg Publications


Sathy BN, Olvera D, Gonzalez-Fernandez T, Cunniffe GM, Pentlavalli S, Chambers P, Jeon O, Alsberg E, McCarthy HO, Dunne N, Donahue TLH, Kelly DJ: RALA complexed α-TCP nanoparticle delivery to mesenchymal stem cells induces bone formation in tissue engineered constructs in vitro and in vivo. Journal of Material Chemistry B (in press, 2017).

Huynh CT, Zheng Z, Nguyen MK; McMillan A, Tonga GY, Rotello V, Alsberg E: Cytocompatible Catalyst-free Photodegradable Hydrogels for Light-Mediated RNA Release to Induce hMSC Osteogenisis. ACS Biomaterials Science and Engineering (in press, 2017). Invited for special themed issue “Tissue Engineering.”


Nguyen MK, McMillan A, Schapira DS, Alsberg E: Photocrosslinkable, biodegradable hydrogels with controlled cell adhesivity for prolonged siRNA delivery to hMSCs to enhance their osteogenic differentiation. Journal of Material Chemistry B (in press, 2016). Invited for special themed issue “Bio-inspired and Natural Materials.”

Ho SS, Vollmer NL, Refaat MI, Jeon O, Alsberg E, Lee MA, Leach JK: Bone morphogenetic protein-2 promotes human mesenchymal stem cell survival and resultant bone formation when entrapped in photocrosslinked alginate hydrogels. Advanced Healthcare Materials (in press, 2016).

Huynh CT, Nguyen MK, Tonga GY, Longe L, Rotello VM, Alsberg E: Photocleavable hydrogels for light-triggered siRNA release. Advanced Healthcare Materials, 5(3):305-10, 2016. Highlighted as cover.

Verrier S, Alini M, Alsberg E, Buchman SR, Kelly D, Laschke MW, Menger MD, Murphy WL, Stegemann JP, Schutz M, Miclau T, Stoddart MJ, Evans C: Tissue engineering and regenerative approaches to improving the healing of large bone defects. eCells and Materials (revised, 2016).

Daly AC, Cunniffe GM, Sathy BN, Jeon O, Alsberg E, Kelly DJ: 3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering. Advanced Healthcare Materials (in press, 2016).

Huynh CT, Nguyen MK, Naris M, Tonga GY, Rotello VM, Alsberg E: Light-Triggered RNA Release and Induction of hMSC Osteogenesis via Photodegradable, Dual-Crosslinked Hydrogels. Nanomedicine (in press, 2016).

Jeon O, Marks R, Wolfson D, Alsberg E: Dual-crosslinked hydrogel microwell system for formation and culture of multicellular human adipose-derived stem cell spheroids. Journal of Material Chemistry B (in press, 2016). Highlighted as inside front cover.

Samorezov JE, Headley EB, Everett CR, Alsberg E: Sustained presentation of BMP-2 enhances osteogenic differentiation of human adipose-derived stem cells in gelatin hydrogels. Journal of Biomedical Materials Research-A, 104(6):1387-97, 2016.

Wang S, Jeon O, Shankles PG, Liu Y, Alsberg E, Retterer ST, Lee BP, Choi CK: In-situ photopolymerization of monodisperse and discoid oxidized methacrylated alginate microgels in a microfluidic channel. Biomicrofluidics, 10(1):011101, 2016.

Dang PN, Dwivedi N, Yu X, Phillips L, Bowerman C, Murphy W, Alsberg E: Guiding chondrogenesis and osteogenesis with mineral-coated hydroxyapatite and BMP-2 incorporated within high-density hMSC aggregates for bone regeneration. ACS Biomaterials (in press, 2016). Highlighted as cover.

Dang PN, Dwivedi N, Phillips L, Yu X, Herberg S, Bowerman C, Solorio LD, Murphy WL, Alsberg E: Controlled growth factor delivery from microparticles incorporated within hMSC aggregates for enhanced bone tissue engineering via endochondral ossification. Stem Cells Translational Medicine, 5(2) 206-17, 2016.


Huynh CT, Nguyen MK, Tonga GY, Longe L, Rotello VM, Alsberg E: Photocleavable hydrogels for light-triggered siRNA release. Advanced Healthcare Materials, 5(3):305-10, 2016. Highlighted as cover.

Solorio LD, Phillips LM, McMillan A, Cheng CW, Dang PN, Samorezov JE, Alsberg E: Spatially Organized Differentiation of Mesenchymal Stem Cells within Biphasic Microparticle-Incorporated High Cell Density Osteochondral Tissues. Advanced Healthcare Materials, 4(15):2306-13, 2015.

Samorezov JE, Morlock CM, Alsberg E: Dual ionic and photocrosslinked alginate hydrogels for micropatterned spatial control of material properties and cell behavior. Bioconjugate Chemistry, 26(7): 1339-47, 2015. Invited for special issue “Biofunctional Biomaterials – The 3rd Generation of Medical Devices.”

Dikina AD, Strobel HA, Lai BP, Rolle MW, Alsberg E: Engineered cartilaginous tubes for tracheal tissue replacement via self-assembly and fusion of human mesenchymal stem cell constructs. Biomaterials, 52: 452-62, 2015.

Jeon O, Wolfson DW, Alsberg E: In situ forming growth factor-loaded coacervate microparticle-embedded hydrogel for directing encapsulated stem cell fate. Advanced Materials, 27(13) 2216-23, 2015. Highlighted as Frontispiece.

Hill MC, Nguyen MK, Jeon O, Alsberg E: Spatial control of cell gene expression by siRNA gradients in biodegradable hydrogels. Advanced Healthcare Materials, 4(5): 714-22, 2015. Highlighted as Back Cover.

Samorezov JE, Alsberg E: Spatial regulation of controlled bioactive factor delivery for bone tissue engineering. Advanced Drug Delivery Reviews, 84: 45-67, 2015.


Boerckel JD, Mason DE, McDermott AM, Alsberg E: Microcomputed tomography: Approaches and applications in bioengineering. Stem Cell Research & Therapy, 5(6): 144, 2014.

Jeong SI, Burns NA, Bonino CA, Kwon IK, Khan SA, Alsberg E: Improved cell infiltration of highly porous 3D nanofibrous scaffolds formed by combined fiber-fiber charge repulsions and ultra-sonication. Journal of Materials Chemistry B, 2(46): 8116-8122, 2014.

Nguyen K, Jeon O, Krebs MD, Alsberg E: Sustained localized presentation of RNA interfering molecules from in situ forming hydrogels to guide stem cell osteogenic differentiation. Biomaterials, 35(24): 6278-86, 2014.

Dang PN, Solorio LD, Alsberg E: Self-assembling human adipose-derived stem cell sheets incorporated with growth factor releasing biodegradable polymer microspheres for cartilage regeneration. Tissue Engineering A, 20(23-24): 3163-75, 2014.

Fu AS, Solorio LD, Alsberg E, Saidel GM: Mathematical modeling of glycosaminoglycan production by stem cell aggregates incorporated with growth factor-releasing polymer microspheres. Journal of Tissue Engineering and Regenerative Medicine (in press, 2014).

Yu X, Dang PD, Alsberg E, Murphy WL: Multilayer inorganic microparticles for tunable dual growth factor delivery. Advanced Functional Materials, 24(20): 3082-3093, 2014.

Nguyen K, Alsberg E: Bioactive factor delivery strategies from engineered polymer hydrogels for therapeutic medicine. Progress in Polymer Science, 39(7): 1236-1265, 2014.

Cheng C, Solorio LD, Alsberg E: Decellularized tissues for orthopaedic tissue engineering. Biotechnology Advances, 32(2):462-84, 2014.

Jeon O, Samorezov J, Alsberg E: Single and dual crosslinked oxidized methacrylated alginate/PEG hydrogels for bioadhesive applications. Acta Biomaterialia, 10(1):47-55, 2014.

Andersen T, Markussen C, Dornish M, Heier-Baardson H, Melvik JE, Alsberg E, Christensen BE: In situ gelation for cell immobilization and culture in alginate foam scaffolds. Tissue Engineering, Part A, 20(3-4)600-10, 2014.

Andersen T, Melvik J, Gåserød O, Alsberg E, Christensen B: Ionically gelled alginate foams: Physical properties controlled by type, amount and source of gelling ions. Carbohydrate Polymers, 99:249-56, 2014.

Heylman CM, Santosa S, Krebs MD, Saidel GM, Alsberg E, Muschler GF: Modeling and experimental methods to predict optimal oxygen distribution for tissue regeneration in bone defects Medical and Biological Computing and Engineering, 52(4):321-30, 2014.


Jeon O, Alt D, Ahmed SM, Linderman S, Alsberg E: Biochemical and physical signal gradients in hydrogels to control stem cell behavior. Advanced Materials, 25(44):6366-72, 2013.

Jeon O, Alsberg E: Regulation of stem cell fate in a three-dimensional micropatterned dual-crosslinked hydrogel system. Advanced Functional Materials, 23(38):4765-75, 2013.

Saquing C, Tang C, Monian B, Bonino C, Manasco J, Alsberg E, Khan S: Alginate-PEO blend nanofibers and the role of carrier polymer in electrospinning. Industrial & Engineering Chemistry Research, 52(26):8692-8704, 2013.

Jeon O, Alsberg E: Photofunctionalization of alginate hydrogels to promote adhesion and proliferation of human mesenchymal stem cells. Tissue Engineering, Part A, 19(11-12):1424-32, 2013.

Corn DJ, Kim Y, Krebs MD, Mounts T, Molter J, Gerson S, Alsberg E, Dennis JE, Lee Z: Imaging early stage osteogenic differentiation of mesenchymal stem cells. Journal of Orthopaedic Research, 31(6):871-9, 2013.

Solorio L, Vieregge E, Dhami C, Alsberg E: High density cell systems incorporating polymer microspheres as microenvironmental regulators in engineering cartilage tissues. Tissue Engineering, Part B, 19(3):209-20, 2013.

Nyugen K, Dang PN, Alsberg E: Functionalized, biodegradable hydrogels for control over sustained and localized siRNA delivery to incorporated and surrounding cells. Acta Biomaterialia, 9(1): 4487-4495, 2013.


Andersen T, Melvik J, Gåserød O, Alsberg E, Christensen B: Ionically Gelled Alginate Foams: Physical Properties Controlled by Operational and Macromolecular Parameters. Biomacromolecules, 13(11): 3703-3710, 2012.

Jeong SI, Jeon O, Krebs MD, Hill MC, Alsberg E: Biodegradable photocrosslinked alginate nanofiber scaffolds with tunable physical properties, cell adhesivity, and growth factor release. eCells and Materials, 24: 331-343, 2012.

Solorio LD, Vieregge EL, Dhami CD, Alsberg: Spatiotemporal regulation of chondrogenic differentiation with controlled delivery of TGF-β1 from gelatin microspheres in mesenchymal stem cell aggregates. Stem Cells Translational Medicine, 1(8): 632-639, 2012.

Chim H, Miller E, Gliniak C, Alsberg E: Stromal-cell-derived factor (SDF) 1-alpha in combination with BMP-2 and TGF-β1 induces site-directed cell homing and osteogenic and chondrogenic differentiation for tissue engineering without the requirement for cell seeding. Cell and Tissue Research, 350(1): 89-94, 2012.

Bonino CA, Efimenko K, Jeong SI, Krebs MD, Alsberg E, Khan SA: Three-Dimensional Electrospun Alginate Mats via Tailored Charge Repulsions. Small, 8(12): 1928-36, 2012.

Jeon O, Alt D, Ahmed SM, Alsberg E: The effect of oxidation on the degradation of photocrosslinkable alginate hydrogels. Biomaterials, 33(13): 3503-3514, 2012.

Solorio LD, Vieregge EL, Dhami CD, Dang PN, Alsberg E: Engineered cartilage via self-assembled hMSC sheets with incorporated biodegradable gelatin microspheres releasing chondrogenic growth factor. Journal of Controlled Release, 158(2): 224-232, 2012.


Bonino CA, Samerozev JE, Jeon O, Alsberg E, Khan SA: Real-time in situ rheology of alginate hydrogel photocrosslinking. Soft Matter, 7(24): 11510-11517, 2011.

Lee JB, Jeong SI, Bae MS, Heo DN, Kim CH, Alsberg E, Kwon IK: Highly porous electrospun nanofibers enhanced by ultra-sonication for improved cellular infiltration. Tissue Engineering Part A, 17(21-22): 2695-2702, 2011.

Jeon O, Powell C, Solorio L, Krebs MD, Alsberg E: Affinity-based growth factor delivery from biodegradable, photocrosslinked heparin-alginate hydrogels.Journal of Controlled Release, 154(3): 258-266, 2011.

Bonino CA, Krebs MD, Saquing CD, Jeong SI, Shearer KL, Alsberg E, Khan SA: Electrospinning alginate-based nanofibers: From blends to crosslinked low molecular weight alginate-only systems. Carbohydrate Polymers, 85(1): 111-119, 2011.

Jeon O, Krebs MD, Alsberg E: Controlled and sustained gene delivery from injectable porous PLGA scaffolds. Journal of Biomedical Materials Research-A,98A(1): 72-79, 2011.

Krebs MD, Alsberg E: Localized, Targeted and Sustained Silencing RNA Delivery.Chemistry - A European Journal,17(11): 3054-3062, 2011. 


Jeong SI, Krebs MD, Bonino CA, Samorezov JE, Khan SA, Alsberg E: Electrospun chitosan-alginate nanofibers with in situ polyelectrolyte complexation for use as tissue engineering scaffolds. Tissue Engineering: Part A, 17(1-2): 59-70, 2010. 

Sasaki JI, Asoh TA, Matsumoto T, Egusa H, Yatani H, Akashi M, Alsberg E, Sohmura T: Fabrication of 3D cell constructs using temperature-responsive hydrogelTissue Engineering: Part A, 16(8): 2497-2504, 2010.

Jeon O, Powell C, Ahmed SM, Alsberg E: Biodegradable, photocrosslinked alginate hydrogels with independently tailorable physical properties and cell adhesivity. Tissue Engineering: Part A, 16(9): 2915-2925, 2010.

Jeong SI, Krebs MD, BoninoCA, KhanSA, AlsbergE: Electrospun alginate nanofibers with controlled cell adhesion for tissue engineering.  Macromolecular Biosciences, 10(8): 934-943, 2010.

Solorio LD, Fu AS, Hernandez-Irizarry R, Alsberg E: Chondrogenic differentiation of human mesenchymal stem cell aggregates via controlled release of TGF-b1 from incorporated polymer microspheres. Journal of Biomedical Materials Research-A, 92(3): 1139-1144, 2010.

Krebs MD, Salter E, Chen E, Sutter KA, Alsberg E: Calcium phosphate-DNA nanoparticle gene delivery from alginate hydrogels induces in vivo osteogenesis.Journal of Biomedical Materials Research-A, 92(3): 1131-1138, 2010.


Erb RM, Krebs MD, Alsberg Em Samata B, Rotello VM, Yellen BB: Beyond diffusion-limited aggregation kinetics in microparticle suspensions. Physical Review E, 80(5 Pt 1), 2009. 

Krebs MD, Jeon O, Alsberg E: Localized and sustained delivery of silencing RNA from macroscopic biopolymer hydrogels. Journal of the American Chemical Society, 131(26): 9204-9206, 2009.

Krebs MD, Erb RM, Yellen BB, Samanta B, Bajaj A, Rotello VM, Alsberg E: Formation of ordered cellular structures in suspension via label-free negative magnetophoresis. Nanoletters, 9(5): 1812-1817, 2009.

Krebs MD, Sutter K, Lin AS, Guldberg RE, Alsberg E: Injectable poly(lactic-co-glycolic) acid scaffolds with in situ pore formation for tissue engineering. ActaBiomaterialia, 5(8): 2847-2759, 2009.

Jeon O, Mansour JM, Alsberg E: Photocrosslinkable alginate hydrogels with tunable biodegradation rates and mechanical properties. Biomaterials, 30(14): 2724-2734, 2009.


Grikscheit TC, Sala FG, Ogilvie J, Bower KA, Ochoa ER, Alsberg E, Mooney DJ, Vacanti JP: Tissue-engineered spleen protects against overwhelming pneumococcal sepsis in a rodent model. Journal of Surgical Research, 149(2): 214-218, 2008.


Matsumoto T, Sasaki JI, Alsberg E, Egusa H, Yatani H, Sohmura T: Three-dimensional cell and tissue patterning in a strained fibrin gel system. PLoS ONE,2(11): e1211, 2007.


Alsberg E, von Recum HA, Mahoney MJ. Environmental cues to guide stem cell fate decision for tissue engineering applications. Expert Opinion on Biological Therapy, 6(9):847-866, 2006.

Alsberg E, Feinstein E, Joy MP, Prentiss M, Ingber DE: Magnetically-guided self-assembly of fibrin matrices with ordered nanoscale structure for tissue engineering. Tissue Engineering, 12(11): 3247-3256, 2006.  (Chosen for cover)

Xia S, Hunt TP, Mayers BT, Alsberg E, Whitesides GM, Westervelt R, Ingber DE. Combined micromagnetic-microfluidic separation of living cells in continuous flow. Biomed. Microdevices, 8(4): 299-308, 2006.


Overby DR, Matthews BD, Alsberg E, Ingber DE: Novel dynamic rheological behavior of individual focal adhesions measured within single cells using electromagnetic pulling cytometry. Acta Biomaterialia, 1(3): 295-303, 2005.

Lee H, Liu Y, Alsberg E, Ingber DE, Westervelt RM, Ham D: An IC/microfluidic hybrid microsystem for 2D magnetic manipulation of individual biological cells.IEEE Int. Solid State Circuits, 80-81, 2005.

Kong HJ, Polte TR, Alsberg E, Mooney DJ: FRET measurements of cell-traction forces and nano-scale clustering of adhesion ligands varied by substrate stiffness. Proceedings of the National Academy of Sciences, 102(12): 4300-4305, 2005.

Moore KA, Polte T, Huang S, Shi B, Alsberg E, Sunday ME, Ingber DE: Control of basement membrane remodeling and epithelial branching morphogenesis in embryonic lung by Rho and cytoskeletal tension. Developmental Dynamics, 232(2): 268-281, 2005.


Lee KY*, Alsberg E*, Hsiong S, Mooney DJ: Nanoscale adhesion ligand organization regulates osteoblast proliferation and differentiation. Nanoletters, 4(8): 1501-1506, 2004. *Shared first authorship.

Kong HJ, Alsberg E, Kaigler D, Lee KY, Mooney DJ: Controlling degradation of hydrogels via size of crosslinked junctions. Advanced Materials, 16(21): 1917-1921, 2004.

Grimmer JF, Gunnlaugsson CB, Alsberg E, Murphy HS, Kong HJ, Mooney DJ, Weatherly RA: Tracheal reconstruction using tissue-engineered cartilage.Archives of Otolaryngology - Head and Neck Surgery, 130(10): 1191-1196, 2004.

Grikscheit TC, Siddique A, Ochoa ER, Srinivasan A, Alsberg E, Holden RA, Vacanti JP: Tissue-engineered small intestine improves recovery after massive small bowel resection. Annals of Surgery, 240(5): 748-754, 2004.

Simmons CA*, Alsberg E*, Hsiong S, Kim WJ, Mooney DJ: Dual growth factor delivery and controlled scaffold degradation enhance in vivo bone formation by transplanted bone marrow stromal cells. Bone, 35(2): 562-569, 2004. *Shared first authorship.

Thornton AJ, Alsberg E, Hill EE, Mooney DJ: Shape retaining injectable hydrogels for minimally invasive bulking. Journal of Urology, 172(2): 763-768, 2004.

Thornton AJ, Alsberg E, Albertelli M, Mooney DJ: Shape-defining scaffolds for minimally invasive tissue engineering. Transplantation, 77(12): 1798-1803, 2004.


Alsberg E, Kong HJ, Hirano Y, Smith MK, Albeiruti A, Mooney DJ: Regulating bone formation via controlled scaffold degradation. Journal of Dental Research,82(11): 903-908, 2003.

Grikscheit TC, Ochoa ER, Ramsanahie A, Alsberg E, Mooney DJ, Whang EE, Vacanti JP: Tissue engineered large intestine resembles native colon with appropriate in vitro physiology and architecture. Annals of Surgery, 238(1): 35-41, 2003.


Alsberg E, Anderson KW, Albeiruti A, Rowley JA, Mooney DJ: Engineering growing tissues. Proceedings of the National Academy of Sciences, 99(19): 12025-12030, 2002.

Grikscheit TC, Ogilvie JB, Ochoa ER, Alsberg E, Mooney DJ, Vacanti, JP: Tissue-engineered colon exhibits function in vivoSurgery, 132(2): 200-204, 2002.


Alsberg E, Anderson KW, Albeiruti A, Franceschi RT, Mooney DJ: Cell-interactive alginate hydrogels for bone tissue engineering. Journal of Dental Research, 80(11): 2025-29, 2001.

Lee KY, Alsberg E, Mooney DJ: Degradable and injectable poly(aldehyde guluronate) hydrogels for bone tissue engineering.  Journal of Biomedical Materials Research-A, 56(2): 228-33, 2001.

Bouhadir KH, Alsberg E, Mooney DJ: Hydrogels for combination delivery of antineoplastic agents. Biomaterials, 22(19): 2625-33, 2001.

Bouhadir KH, Lee KY, Alsberg E, Damm KL, Anderson KW, Mooney DJ: Degradation of partially oxidized alginate and its potential application for tissue engineering. Biotechnology Progress, 17(5): 945-50, 2001.

Alsberg E, Hill EE, Mooney DJ: Craniofacial tissue engineering. Critical reviews in oral biology and medicine, 12(1): 64-75, 2001.


Brown AN, Kim BS, Alsberg E, Mooney DJ: Combining chondrocytes and smooth muscle cells to engineer hybrid soft tissue constructs. Tissue Engineering, 6(4): 297-305, 2000.


Richards M, Wineman AS, Alsberg E, Goulet JA, Goldstein SA: Viscoelastic characterization of mesenchymal gap tissue and consequences for tension accumulation during distraction. J. Biomech. Eng., 121: 116-123, 1999.


Book Chapters


Nguyen K, Samorezov J, Alsberg E. Hydrogels in bone tissue engineering. In: Khademhosseini A, et al., eds. Applications of Hydrogels in Regenerative Medicine (in press, 2014).


Lee Z, Dennis J, Alsberg E, Krebs MD, Welter J, Caplan A: Imaging stem cell differentiation for cell-based tissue repair. Methods Enzymol., 506:247-63, 2012.

Andersen T, Strand BL, Formo K, Alsberg E, Christensen BE. Alginates as biomaterials in tissue engineering. In: Rauter AP, ed. Carbohydrate Chemistry Vol. 37: Chemical and Biological Approaches – Glycosciences for Human Health and Disease. London, England: Royal Society of Chemistry, 2012: 227-258.


Alsberg E, Moore K, Huang S, Polte T, Ingber DE. The mechanical and cytoskeletal basis of lung morphogenesis. In: Massaro D, et al., eds. Lung Development and Regeneration. Vol. 190. New York, NY: Marcel Dekker, Inc., 2004: 247-274.