Olympus FV1000 Confocal Microscope

Confocal microscopy overcomes the diffraction limit of lateral resolution inherent to conventional light-optical microscopy, and can deliver blur-free images of thick specimens, suitable for 3D reconstruction.
The Olympus BX62 confocal microscope provides a broad variety of optical imaging modes:
  • Laser confocal imaging
  • Bright-field imaging.
  • Dark-field imaging.
  • Nomarski DIC (differential interference contrast).
  • Epiflourescence.

For the conventional imaging modes, a mercury lamp can be used for reflected light or epifluorescence imaging. A halogen lamp is available for transmitted light work. A variety of high quality objectives are available. Oil immersion objectives are available for the highest resolution imaging. In the laser scanning mode, six laser lines are available for imaging or fluorescence stimulation: 405, 458, 488, 515, 543, and 633 nm. Three imaging channels are available in reflected/fluoresced light mode, as well as one transmitted channel. A three-axis motorized stage allows for fully automated imaging. Two Linkham purpose specialty stages are available, a TS1500 hot stage, which can operate at temperatures up to 1500 °C and a TST350 tensile stage which can operate from -196° to 350°C.